Microbiology resource announcements
Authors: Retallack H, Clubb S, DeRisi JL
BMC nephrology
Authors: Kim ED, Watt J, Tereshchenko LG, Jaar BG, Sozio SM, Kao WHL, Estrella MM, Parekh RS
The Science of the total environment
Authors: Hill LD, Pillarisetti A, Delapena S, Garland C, Pennise D, Pelletreau A, Koetting P, Motmans T, Vongnakhone K, Khammavong C, Boatman MR, Balmes J, Hubbard A, Smith KR
Case reports in cardiology
Authors: DesJardin JT, Zier LS
PLoS pathogens
Authors: Chang KM, Traum D, Park JJ, Ho S, Ojiro K, Wong DK, Wahed AS, Terrault NA, Khalili M, Sterling RK, Janssen HLA, Shuhart MC, Lau DT, Roberts LR, Johnson GS, Kaplan DE, Betts MR, Lee WM, Lok ASF
JCI insight
Authors: Schaefer N, Li X, Seibold MA, Jarjour NN, Denlinger LC, Castro M, Coverstone AM, Teague WG, Boomer J, Bleecker ER, Meyers DA, Moore WC, Hawkins GA, Fahy J, Phillips BR, Mauger DT, Dakhama A, Gellatly S, Pavelka N, Berman R, Di YP, Wenzel SE, Chu HW
Volume 364 of Issue 6437 | Science (New York, N.Y.)
Authors: Wienert B, Wyman SK, Richardson CD, Yeh CD, Akcakaya P, Porritt MJ, Morlock M, Vu JT, Kazane KR, Watry HL, Judge LM, Conklin BR, Maresca M, Corn JE
CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target sites. Current off-target discovery methods work using purified DNA or specific cellular models but are incapable of direct detection in vivo. We developed DISCOVER-Seq (discovery of in situ Cas off-targets and verification by sequencing), a universally applicable approach for unbiased off-target identification that leverages the recruitment of DNA repair factors in cells and organisms. Tracking the precise recruitment of MRE11 uncovers the molecular nature of Cas activity in cells with single-base resolution. DISCOVER-Seq works with multiple guide RNA formats and types of Cas enzymes, allowing characterization of new editing tools. Off-targets can be identified in cell lines and patient-derived induced pluripotent stem cells and during adenoviral editing of mice, paving the way for in situ off-target discovery within individual patient genotypes during therapeutic genome editing.
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Clinical genitourinary cancer
Authors: Channing J. Paller, Emmanuel S. Antonarakis, Tomasz M. Beer, Hala T. Borno, Maria I. Carlo, Daniel J. George, Julie N. Graff, Shilpa Gupta, Elisabeth I. Heath, Celestia S. Higano, Rana R. McKay, Alicia K. Morgans, Akash Patnaik, Daniel P. Petrylak, Matthew B. Rettig, Charles J. Ryan, Mary-Ellen Taplin, Young E. Whang, Jacob Vinson, Heather H. Cheng, Veda N. Giri
Nature
Authors: Shi Y, Gao W, Lytle NK, Huang P, Yuan X, Dann AM, Ridinger-Saison M, DelGiorno KE, Antal CE, Liang G, Atkins AR, Erikson G, Sun H, Meisenhelder J, Terenziani E, Woo G, Fang L, Santisakultarm TP, Manor U, Xu R, Becerra CR, Borazanci E, Von Hoff DD, Grandgenett PM, Hollingsworth MA, Leblanc M, Umetsu SE, Collisson EA, Scadeng M, Lowy AM, Donahue TR, Reya T, Downes M, Evans RM, Wahl GM, Pawson T, Tian R, Hunter T
JMIR research protocols
Authors: Kittelson S, Scarton L, Barker P, Hauser J, O'Mahony S, Rabow M, Delgado Guay M, Quest TE, Emanuel L, Fitchett G, Handzo G, Yao Y, Chochinov HM, Wilkie D
