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Department of Medicine faculty members published more than 3,000 peer-reviewed articles in 2022.

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Volume 60 of Issue 10 | Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Inflammation inhibits GPR81 expression in adipose tissue.

2011

Authors: Feingold KR, Moser A, Shigenaga JK, Grunfeld C

View Abstract

OBJECTIVE AND DESIGN

The aim of this study was to examine the expression of G protein-coupled receptor 81 (GPR81) in mouse adipose tissue in response to inflammatory stimuli. GPR81 is activated by lactate resulting in the inhibition of lipolysis.

MATERIALS AND TREATMENT

Mice were injected with saline, lipopolysaccharide (LPS), zymosan, or turpentine, N = 5 per group. 3T3-L1 adipocytes were treated with tumor necrosis factor alpha, interleukin (IL)-l beta, IL-6, or interferon gamma.

METHODS

GPR81 expression levels were measured by real-time PCR and statistical significance was determined by Student's t test.

RESULTS

LPS resulted in a marked decrease in GPR81 mRNA level in mouse adipose tissue in C57BL/6 and OuJ mice, an effect that was not observed in HeJ mice, which have a mutation in TLR4. Zymosan and turpentine also decreased adipose tissue GPR81 expression. Cytokine treatment of 3T3-L1 adipocytes had no effect on GPR81 expression. GPR81 expression was decreased in ob/ob mice, an animal model of type 2 diabetes that is characterized by inflammation.